Welcome

Daphnia DB is a freely accessible Daphnia mutant database website that allows you to explore available Daphnia mutant lines and associated genomic and phenotype data. 

Here you are able to place orders for Daphnia mutant lines, find gene knock-out experimental protocols, training materials, and reagents (e.g., plasmid libraries).

Available Tools

There are several tools available for analysis...

SequenceServer BLAST

SequenceServer logo

SequenceServer helps identify unique sequence regions in viruses, bacteria or other target organisms that can serve as diagnostic markers for disease detection.

SequenceServer helps identify unique sequence regions in viruses, bacteria or other target organisms that can serve as diagnostic markers for disease detection. Design or PCR or qPCR primers and probes, or specific guide RNA oligos (gRNA) for Crispr/Cas9 assays. Improving specificity and sensitivity of molecular-genomic approaches supports disease surveillance and management efforts.

crispRdesignR

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Software used to design guide RNA sequences for CRISPR/Cas9 genome editing

This package aims to provide scientifically pertinent information when designing guide RNA sequences for Cas9 genome editing. When provided a target DNA sequence for editing, a genome to check for off-targets in, and a genome annotation file (.gtf), it will output information for two separate data tables. 

The first table contains information on the generated sgRNA (sgRNA sequence, PAM, Direction, Start, End, GC content, Presence of Homopolymers, Regions of Self Complementarity, Effciency Score (Doench 2016), Number of Potential Off-Target sequences, and Notes on the sgRNA). 

The second table contains information on the found off-target sequences (Original sgRNA Sequence, Chromosome, Start, End, Number of Mismatches, Direction, CFD Scores, Matched Sequence, Gene ID, Gene Name, Sequence Type, and Exon Number). 

JBrowse

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JBrowse is a new kind of genome browser that runs on the web, on your desktop, or embedded in your app.

Features include improved structural variant and comparative genomics visualization with linear, circular, dotplot, and synteny views. There is support for many common data types including BAM, CRAM, tabix indexed VCF, GFF, BED, BigBed, BigWig, and several specialized formats. Additionally, there is endless extensibility with a plugin ecosytem which can add additional view types, track types, data adapters, and more! See a summary of new features and a comparison to JBrowse 1